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# Program: MUSTANG-Lite v0.1: A  Multiple structural alignment algorithm
# Authors: A. S. Konagurthu, A. M. Lesk, J. C. Whisstock, and P. J. Stuckey
# Rundate: Fri Jul 22 20:45:31 2005
# Report_file: /mnt/EXTRA_STORAGE/MUSTANG_RESULTS/HOMSTRAD_Datasets/results/isoamylase_NC.html
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#====================================
# Aligned_structures: 2
#   1: 1bf2.pdb
#   2: 1ehaa.pdb
#
# Length:        824
# Identity:       93/824 ( 11.3%)  (Calculated as the percentage of conserved columns in the alignment.)
# Similarity:     93/824 ( 11.3%)  (Calculated as the percentage of semi-conserved columns in the alignment)
# Gaps:          341/824 ( 41.4%)  (Calculated as the percentage of columns with atleast one gap.)

#===========================================ALIGNMENT START=========================================


1bf2.pdb                1  AINSM-SLGASYDAQQANITFRVYSSQATRIVLYLYSAGYGVQESATYTLSPAGSGVWAV   59
1ehaa.pdb               1  -----TFA-YKID--GNEVIFTLWAPYQKSVKLKVLE-------KGLYEMERDEKGYFTI   45
                                       D       F           L              Y       G    

1bf2.pdb               60  TVPVSSIKAAGITGAVYYGYRAWGPNWPYASNWGKGSQAGFVSDVDANGDRFNPNKLLLD  119
1ehaa.pdb              46  TLNN-------VKV--RDRYKYVLD----------------------------DASEIPD   68
                           T                  Y                                       D

1bf2.pdb              120  PYAQEVSQDPLNPSNQNGNVFASGASYRTTDSGIYAPKGVVLVPSTQSTGTKPTRAQKDD  179
1ehaa.pdb              69  PASRYQPEG------------------------V-HGPSQIIQESKEFNNE-TFLKKEDL  102
                           P                                           S             D 

1bf2.pdb              180  VIYEVHVRGFTEQDTSIPAQYRGTYYGAGLKASYLASLGVTAVEFLPVQETQNDANDVVP  239
1ehaa.pdb             103  IIYEIHVGTFTP---------EGTFEGVIRKLDYLKDLGITAIEIMPIAQFP--------  145
                            IYE HV  FT           GT  G   K  YL  LG TA E  P             

1bf2.pdb              240  NSDAN-QNYWGYMTENYFSPDRRYAYNKAAGGPTAEFQAMVQAFHNAGIKVYMDVVYNHT  298
1ehaa.pdb             146  -----GKRDWGYDGVYLYAVQNSYG-------GPEGFRKLVDEAHKKGLGVILDVVYNHV  193
                                    WGY           Y            F   V   H  G  V  DVVYNH 

1bf2.pdb              299  AE-GGTWTSSDPTTATIY-SWRGLDNATYYELTS--GNQYFYDNTGIGANFNTY---NTV  351
1ehaa.pdb             194  GPEG--------------NYMVKLGP--------YFSQ-KY--KTPWGLTFNFDDAESDE  228
                              G                   L                    T  G  FN        

1bf2.pdb              352  AQNLIVDSLAYWANTMGVDGFRFDLASVLGNSCLNGAYTASAPNCPNGGYNFDAADS--N  409
1ehaa.pdb             229  VRKFILENVEYWIKEYNVDGFRLDAVHAII-DT------------------------SPK  263
                               I     YW     VDGFR D                                    

1bf2.pdb              410  VAINRILREFTVRPAAGGSGLDLFAEPWAIGGNSYQLGGF-P-------QGWSEWNGLFR  461
1ehaa.pdb             264  HILEEIADVVH-----KY-NRIVIAESD-L----NDPRVVNPKEKVGYNI-DAQWVDDFH  311
                                I                  AE               P            W   F 

1bf2.pdb              462  DSLRQAQNE------LGSMTIYVTQDANDFSG-S-------SNLFQ------SSGRSPWN  501
1ehaa.pdb             312  HSIHAYLTGERQGYYTDF--GNLDDIVKSYKDVFVYDGKYSNFRR-KTHGEPVGELDGCN  368
                            S                                                         N

1bf2.pdb              502  SINFIDVHDGMTLKDVYSCNGANNSQAWPYGPSDGGTSTNYSWDQGMSAGTGAAVD----  557
1ehaa.pdb             369  FVVYIQNHDQV---GNRG--KGER--------------------------------IIKL  391
                               I  HD                                                   

1bf2.pdb              558  -QRRAARTGMAFEMLSAGTPLMQGGDEYLRTLQC--------------------------  590
1ehaa.pdb             392  VDRESYKIAAALYLLSPYIPMIFMGEEYGEEN--PFYFFSDFSDSKLIQGVREGRKKENG  449
                             R       A   LS   P    G EY                                

1bf2.pdb              591  NNNAYNLD--SSANWLTYSWTTDQS-NFYTFAQRLIAFRKAHPALRPSSWYSGSQLTWYQ  647
1ehaa.pdb             450  QDTDPQDESTFNASKLSWKI----DEEIFSFYKILIKMRKELSIA--CDRRV--NVVNG-  500
                                       A  L              F   LI  RK                    

1bf2.pdb              648  PSGAVADSNYWNNTSNYAIAYAINGPSLGDSNSIYVAYNGWSSSVTFTLPAPPSGTQWYR  707
1ehaa.pdb             501  ---------------ENWLIIKGR--------EYFSLYVFSKSS--IEVKY-----SGTL  530
                                                                Y    SS                

1bf2.pdb              708  VTDTCDWNDGASTFVAPGSE-TLIGGAGTTYGQCGQSLLLLISK  750
1ehaa.pdb             531  LLSSN------NSF------PQHIEE--GKYE-FDKGFALYK--  557
                                        F         I      Y        L    


#=========================================ALIGNMENT END=============================================
#LEGEND:
#
# Colours indicate the chemical nature of the amino acid;
# Red         = small hydrophobic including aromatic,{A,F,I,L,M,P,V,W}
# Blue        = Acidic,{D,E}
# Magenta     = Basic,{K,R} and
# Green       = Basic amino acids with hydroxyl groups and/or amine groups {C,G,H,N,Q,S,T,Y}.
#
# The "markup row" below each stretch of the multiple alignment is used to mark completely conserved
# residue (denoted in UPPERCASE) and semi-conserved reside ( denoted in lowercase) in a column of the alignment.
#
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